HPLC. A Practical Guide, ANALITYCZNA
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HPLC
A
Practical
Guide
RSC
Chromatography Monographs
Series Editor: Roger M. Smith,
Loughborough University,
Loughborough,
UK
Advisory Panel:
J.C.
Berridge,
Sandwich,
UK;
G.B. Cox,
Illkirch, France;
I.S.
Lurie,
Virginia, USA;
P.J. Schoenmaker,
Amsterdam, The Netherlands;
C.F. Simpson,
London,
UE,
G.G.
Wallace,
Wollongong, Australia.
This series is designed for the individual practising chromatographer, providing
guidance and advice on a wide range
of
chromatographic techniques with the
emphasis
on
important practical aspects of the subject.
Packed Column SFC
by
T.A.
Berger,
Berger Instruments, Newark,
Delaware, USA
Chromatographic Integration Methods, Second Edition
by Norman Dyson,
Dyson Instruments Ltd, UK
Separation
of
Fullerenes by Liquid Chromatography
edited by
IS.
Jinno,
Toyohashi University
of
Technology, Japan
HPLC:
A
Practical Guide
by T. Hanai,
Health Research Foundation, Kyoto, Japan
How to obtain future titles on publication
A
standing order plan is available for this series.
A
standing order will bring delivery of each new
volume immediately upon publication.
For
further information, please write to:
The Royal Society of Chemistry, Turpin Distribution Services Ltd., Blackhorse Road, Letchworth,
Hertfordshire
SG6
1
HN,
UK.
Telephone: Letchworth +44(0) 1462 672555: Fax:
+
(44)O
1462
480947
CHROMATOGRAPHY
MONOGRAPHS
HPLC
A
Practical
Guide
T. Hanai
Health Research Foundation, Kyoto, Japan
RSC
ROYAL SOCIETY OF CHEMISTRY
ISBN 0-85404-515-5
A
catalogue record for this book
is
available from the British Library
0
The Royal Society of Chemistry 1999
All rights reserved.
Apart from any fair dealing for the purposes
of
research or private study, or criticism or
review as permitted under the terms of the UK Copyright, Designs and Patents Act, 1988,
this publication may not be reproduced, stored or transmitted, in anyform or by any means,
without the prior permission in writing of The Royal Society of Chemistry, in the case of
reprographic reproduction only in accordance with the terms
of
the licences issued by the
Copyright Licensing Agency in the
UK,
or in accordance with the terms
of
the licences
issued by the appropriate Reproduction Rights Organization outside the UK. Enquiries
concerning reproduction outside the terms stated here should be sent to The Royal Society
of
Chemistry at the address printed on this page.
Published by The Royal Society of Chemistry,
Thomas Graham House, Science Park, Milton Road
Cambridge CB4
OWF, UK
For further information see our web site at www.rsc.org
Typeset by Paston PrePress Ltd, Beccles, Suffolk
Printed by Bookcraft (Bath) Ltd
Preface
high-performance liquid
be separated and on why
particular compounds are separated by liquid chromatographic methods. It
consists of six chapters: Basic Concepts of HPLC; Instrumentation: Prepara-
tion, Testing and Selectivity of Stationary Phase Materials; Selection of the
Eluent; Separation Based on an Improved Column Efficiency; Influence of
Physical Chemistry on Separations in Liquid Chromatography. The focus is on
the basic considerations in liquid chromatography rather than the applications
but there are also sections on trouble-shooting. The book is concerned mainly
with the selection of a packing material and the preparation of the eluent.
Theoretical optimization is demonstrated using the chromatography of simple
chemicals, to aid the understanding of liquid chromatography by graduate
students. The examples given go beyond what can be found in general textbooks
on liquid chromatography. The separations can be easily understood from the
differences in properties of familiar compounds known to undergraduate
students.
The chromatographic separations described are based on solubility using
molecular properties. The selection of chromatographic mode and column is
analysed using solubility parameters. The separation factor
cc
is described in
detail by physical and chemical parameters. Discussion of instrumentation
focuses on high-efficiency operation and is concerned with degassing, the time
constant,
flow
cell design, connectors and trouble-shooting. Packing materials
and their synthesis and surface modification, liquid chromatographic analysis
of surface activity and the evaluation of packed columns, including the
measurement of void volume, are also considered.
I thank Professor Roger Smith, of Loughborough University, who reviewed
the original manuscript and offered many helpful suggestions. I also deeply
appreciate the support of The Royal Society of Chemistry for this publication.
Toshihiko Hanai, Yokohama
V
This book answers two basic questions regarding
chromatography; it focuses on how compounds can
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zanotowane.pl doc.pisz.pl pdf.pisz.pl chiara76.opx.pl
HPLC
A
Practical
Guide
RSC
Chromatography Monographs
Series Editor: Roger M. Smith,
Loughborough University,
Loughborough,
UK
Advisory Panel:
J.C.
Berridge,
Sandwich,
UK;
G.B. Cox,
Illkirch, France;
I.S.
Lurie,
Virginia, USA;
P.J. Schoenmaker,
Amsterdam, The Netherlands;
C.F. Simpson,
London,
UE,
G.G.
Wallace,
Wollongong, Australia.
This series is designed for the individual practising chromatographer, providing
guidance and advice on a wide range
of
chromatographic techniques with the
emphasis
on
important practical aspects of the subject.
Packed Column SFC
by
T.A.
Berger,
Berger Instruments, Newark,
Delaware, USA
Chromatographic Integration Methods, Second Edition
by Norman Dyson,
Dyson Instruments Ltd, UK
Separation
of
Fullerenes by Liquid Chromatography
edited by
IS.
Jinno,
Toyohashi University
of
Technology, Japan
HPLC:
A
Practical Guide
by T. Hanai,
Health Research Foundation, Kyoto, Japan
How to obtain future titles on publication
A
standing order plan is available for this series.
A
standing order will bring delivery of each new
volume immediately upon publication.
For
further information, please write to:
The Royal Society of Chemistry, Turpin Distribution Services Ltd., Blackhorse Road, Letchworth,
Hertfordshire
SG6
1
HN,
UK.
Telephone: Letchworth +44(0) 1462 672555: Fax:
+
(44)O
1462
480947
CHROMATOGRAPHY
MONOGRAPHS
HPLC
A
Practical
Guide
T. Hanai
Health Research Foundation, Kyoto, Japan
RSC
ROYAL SOCIETY OF CHEMISTRY
ISBN 0-85404-515-5
A
catalogue record for this book
is
available from the British Library
0
The Royal Society of Chemistry 1999
All rights reserved.
Apart from any fair dealing for the purposes
of
research or private study, or criticism or
review as permitted under the terms of the UK Copyright, Designs and Patents Act, 1988,
this publication may not be reproduced, stored or transmitted, in anyform or by any means,
without the prior permission in writing of The Royal Society of Chemistry, in the case of
reprographic reproduction only in accordance with the terms
of
the licences issued by the
Copyright Licensing Agency in the
UK,
or in accordance with the terms
of
the licences
issued by the appropriate Reproduction Rights Organization outside the UK. Enquiries
concerning reproduction outside the terms stated here should be sent to The Royal Society
of
Chemistry at the address printed on this page.
Published by The Royal Society of Chemistry,
Thomas Graham House, Science Park, Milton Road
Cambridge CB4
OWF, UK
For further information see our web site at www.rsc.org
Typeset by Paston PrePress Ltd, Beccles, Suffolk
Printed by Bookcraft (Bath) Ltd
Preface
high-performance liquid
be separated and on why
particular compounds are separated by liquid chromatographic methods. It
consists of six chapters: Basic Concepts of HPLC; Instrumentation: Prepara-
tion, Testing and Selectivity of Stationary Phase Materials; Selection of the
Eluent; Separation Based on an Improved Column Efficiency; Influence of
Physical Chemistry on Separations in Liquid Chromatography. The focus is on
the basic considerations in liquid chromatography rather than the applications
but there are also sections on trouble-shooting. The book is concerned mainly
with the selection of a packing material and the preparation of the eluent.
Theoretical optimization is demonstrated using the chromatography of simple
chemicals, to aid the understanding of liquid chromatography by graduate
students. The examples given go beyond what can be found in general textbooks
on liquid chromatography. The separations can be easily understood from the
differences in properties of familiar compounds known to undergraduate
students.
The chromatographic separations described are based on solubility using
molecular properties. The selection of chromatographic mode and column is
analysed using solubility parameters. The separation factor
cc
is described in
detail by physical and chemical parameters. Discussion of instrumentation
focuses on high-efficiency operation and is concerned with degassing, the time
constant,
flow
cell design, connectors and trouble-shooting. Packing materials
and their synthesis and surface modification, liquid chromatographic analysis
of surface activity and the evaluation of packed columns, including the
measurement of void volume, are also considered.
I thank Professor Roger Smith, of Loughborough University, who reviewed
the original manuscript and offered many helpful suggestions. I also deeply
appreciate the support of The Royal Society of Chemistry for this publication.
Toshihiko Hanai, Yokohama
V
This book answers two basic questions regarding
chromatography; it focuses on how compounds can
[ Pobierz całość w formacie PDF ]